Thin Layer Chromatography (TLC): An Introduction

Written By Adeel Abbas What is Thin Layer Chromatography? Thin layer chromatography (TLC) is a widely used separation technique in analytical chemistry that is used to separate and identify different compounds present in a mixture. TLC is a quick and easy-to-use method that is used for qualitative and quantitative analysis of a wide range of … Read more

Ascending and Descending Chromatography: A Brief Overview

Written by Adeel Abbas Chromatography is a powerful analytical technique used in various fields, including chemistry, biochemistry, and pharmaceuticals. It involves separating and identifying components of a mixture based on their physical and chemical properties. There are different types of chromatography techniques, including ascending and descending chromatography. In this article, we will provide a brief … Read more

Low-Pressure Liquid Chromatography (LPLC)-A detailed guide

Written by Adeel Abbas In the world of analytical chemistry, low-pressure liquid chromatography (LPLC) is a widely used technique for separating and analyzing biomolecules, like proteins, peptides, and monoclonal antibodies. It is a cost-effective and efficient method that utilizes a column packed with stationary phase particles to separate components based on their differing physical and … Read more

High-Performance Liquid Chromatography (HPLC)- A detailed guide

Written by Adeel Abbas What is High-Performance Liquid Chromatography (HPLC)? High-Performance Liquid Chromatography (HPLC) is an analytical technique used to separate, identify, and quantify components in a mixture. It is a powerful tool that has become an indispensable part of modern analytical chemistry. HPLC is based on the principles of liquid chromatography, which involves the … Read more

10 Examples of Mixtures that Can be Separated by Chromatography

Chromatography is a powerful separation technique that is widely used in a variety of fields, including chemistry, biochemistry, pharmaceuticals, and forensics. A chromatography method for separating mixtures into their individual components. In this method, a mixture is separated into its different components based on their different interactions with a stationary phase and mobile phase. These … Read more

Column Chromatography-Principle, Types, Applications

Written By Adeel Abbas Column chromatography is a widely used technique of chromatography in chemistry to separate and purify a mixture of chemical compounds. Mostly it is used in natural product isolation. I also used this technique during my research on Boerhavia Procumbens. The goal of this technique is to isolate each component in a … Read more

Gas chromatography-intro, principle, working, applications

image showing gas chromatography diagram

Written By Adeel Abbas Gas Chromatography (GC) is a powerful analytical technique used to separate and identify individual components in a mixture of gases or volatile liquids. The technique of chromatography is widely used in a variety of fields, including chemistry, environmental science, and forensic science. Gas chromatography is a method of separation in which … Read more

Chromatography-Principle, types, uses and History

Written by Adeel Abbas Chromatography is a technique used to separate a mixture of chemical compounds into its components.   Definition of chromatography Imagine you have a jar of mixed colored candies, red, blue and green. And you want to separate the candies based on their colors. One way you could do this is by … Read more